Split MR model with Slice-n-Dice

Part of Slice-n-Dice automated MR pipeline used to slice predicted models into distinct structural units which are then can be placed in an automatic MR pipeline.

Slice option in CCP4 cloud uses Birch clustering algorithm based on the coordinates of the Cɑ atoms.

The number of splits (clusters) can be specified. The large target protein needs to be modeled in several chunks. An increasing number of splits allows slice and dice to test a range of different splits.

The default pLDDT threshold for AlphaFold2 models is 70 and the default RMS threshold for RosettaFold models is 1.75.

ACKNOWLEDGEMENTS

This article uses materials kindly provided by Dr. Adam Simpkin and Dr. Ronan Keegan, whose help is greatly appreciated.